Research on creating a recombinant cell line to produce peptides from frog skin with antibacterial properties
The objective is to select a peptide derived from frog skin that has the ability to kill specific pathogenic microorganisms, drug-resistant microorganisms, and anti-cancer cells, and at the same time, create a recombinant cell line that has synthesis capable of producing selected peptides with a peptide content of 120mg/g dry cells, and building a fermentation process and a model of a peptide-producing recombinant cell line with the sale of 100 liter/batch, purified and obtained with active peptide. The research team investigated the peptides of some common frog species in Vietnam, designed genes encoding polypeptides and created microbial cell lines capable of expressing recombinant polypeptides.
Overview of the acceptance session of the project "Research on creating a recombinant cell line to produce peptides from frog skin with antibacterial properties to replace antibiotics and support cancer treatment"
Specifically, the research team collected and classified 30 frog samples belonging to 11 different frog species, cloned 10 gene sequences coding for 10 different peptides, thereby creating four animal cell lines with different abilities to express recombinant polypeptide and conduct evaluation. Dr. La Thi Huyen, Project leader, said: "Through research on AMP peptides on some common frog species in Vietnam, the research team designed genes encoding polypeptides and created microbial cell lines that are able to express recombinant polypeptides. From there, creating an animal cell line capable of expressing recombinant polypeptide and conducting evaluation. As a result, the obtained recombinant peptide has a purity of over 80%, meeting the prescribed quality standards, which can be used to replace antibiotics and support cancer treatment."
Dr. La Thi Huyen, Project leader, made a presentation at the acceptance session
The project has studied recombinant cell lines capable of synthesizing peptides with antibacterial and anti-cancer properties with 100% of cells carrying genes encoding target peptides. Along with that, the project has studied peptides produced from frog skin reaching 120 mg/g of dry cells, which are resistant to pathogenic microorganisms and cancer cells. Notably, the recombinant peptide has been studied with peptide purity 8.13 > 83.6%, peptide purity BrevininKK > 85.5%, meeting the prescribed quality standards, used as an alternative to antibiotics and support cancer treatment. The products all met or exceeded the quantity and quality targets compared with the contract signed with the Ministry of Industry and Trade.
Within the framework of the project, the research team also built a technological process to create a recombinant cell line to produce peptides from frog skin on a scale of 100 liters of fermented juice/batch. In addition, a model of a recombinant cell line generation device was built to produce peptides from frog skin on a scale of 100 liters of fermented juice/batch, and a socio-economic efficiency assessment report and report on the application or technology transfer into production practice were completed. The group has also completed 1 scientific article, trained 1 master and registered 1 useful solution that was accepted by the National Office of Intellectual Property.
Food products to protect the health of the project
The acceptance council appreciated the efforts that the research team has made. Assoc. Dr. Nguyen Thi Hoai Tram, Chairman of the Council, said that the project has completed the research contents and objectives, creating recombinant cell lines capable of synthesizing peptides and technological processes. The products all meet the quantity and quality targets compared with the signed contract. However, it is necessary to continue to edit, supplement and complete the summary report, continue to research and apply, and soon put the research results into practice.
Translated by Tuyet Nhung
Link to Vietnamese version