Simulated microgravity inhibits mesenchymal stem cell proliferation and remodeling

The cytoskeleton plays a very important role in cell proliferation, helping to maintain cell shape and internal organization. Cells are very sensitive to changes in simulated microgravity (SMG) conditions. SMG can induce changes in skeletal distribution and cell proliferation.

Dr. Le Thanh Long and colleagues at Institute of Tropical Biology have studied and evaluated the effects of simulated microgravity on proliferation and the expression of key cell cycle-related regulatory factors and skeletal structural proteins in human umbilical cord mesenchymal stem cells (hucMSCs).

The WST-1 test results showed that the proliferation level of hucMSC cells was lower in the SMG group than in the control group. Furthermore, flow cytometry analysis demonstrated that the percentage of hucMSCs in the SMG group during the G0/G1 period was higher than in the control group. Western blot analysis showed a decrease in the expression of cyclin A1 and A2 in the hucMSC group under SMG conditions. The expression of proteins involved in cell cycle regulation cdk4 and cdk6 also decreased in hucMSCs in the SMG group. The total nucleation intensity of hucMSCs in the SMG group was lower than in the control group. However, there was no difference in nucleus area or nucleus shape value of hucMSC in the two experimental groups. Realtime qRT-PCR and Western blot analysis results both showed that hucMSCs in the SMG group reduced β-actin and α-tubulin 3 expression compared to the control group. SMG conditions also induce microtubule and microfilament reorganization in hucMSCs. The study showed that decreased expression of key cell cycle-related proteins and cytoskeleton structural proteins resulted in remodeling of the cytoskeleton and decreased proliferation in hucMSCs.

The above research results belong to the project: "Research and evaluation of changes in the structure of the skeleton of living cells under simulated microgravity conditions", code: VT-CB.15 /18-20 under the National Science and Technology Program on Space Technology (2016-2020), directed by Dr. Le Thanh Long, chaired by Institute of Tropical Biology.

Changes in hucMSC cells under SMG conditions

A: WST-1 analysis. B: flow cytometry. C, D: cell cycle-related protein expression and microtubule and microfilament structure. E: mRNA expression analysis of actin and tubulin genes

F, G: microtubules and microfilaments in hucMSC cells

Translated by Phuong Ha
Link to Vietnamese version

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